Instructions for authors

Abstracts should be prepared in Word format (Arial, with 10 pt characters) and sent online through the Meeting website (SUBMIT). The deadline for abstract submission will be March 15th at 23:59h.

Authors must submit the abstracts in English providing the following information:

Title: should be in bold. Max. 200 characters (incluiding spaces).

Author(s): include the complete names of all authors, i. e. James S. Wright, Geoffrey E. Grant. The name of the presenting author should be underlined.

Affiliation: List of institutions that participate in the study indicating the affiliation of each author.

Abstract text (incl. references): max 3500 characters (including spaces). Abstracts should include only text (figures, images and formulas are not allowed) and should describe the objectives, results and conclusions.

Reference style
References should be cited in the text by number in parentheses only and in order of appearance. The examples provided below should be followed.
Lopez JJ, Albarrán L, Jardín I, Sanchez-Collado J, Redondo PC, Bermejo N, Bobe R, Smani T, Rosado JA. (2018) Filamin A modulates store-operated Ca2+ entry by regulating STIM1-Orai1 association in human platelets. Arterioscler Thromb Vasc Biol. 38(2): 386-397.

Jardin I, Diez-Bello R, Lopez JJ, Redondo PC, Salido GM, Smani T, Rosado JA. (2018) TRPC6 channels are required for proliferation, migration and invasion of breast cancer cell lines by modulation of Orai1 and Orai3 surface exposure. Cancers (Basel). 10(9): 331.

Schindl R, Fahrner M, Muik M, Romanin R. (2012) The STIM-Orai pathway. The interactions between STIM1 and Orai1. In: Store-operated Ca2+ entry (SOCE) pathways, ed. Groschner K, Graier W, Romanin R, pp. 45-56. Springer, New York.

Keywords: should include at least five keywords separated by semicolons.

Acknowledgements: Authors might acknowledge the funding bodies and the individuals who provided help during the research.
There is no limit for the number of abstracts that a researcher can sign as an author; however, an author can only present a maximum of two communications.

To be accepted, at least one of the authors of the communication must be registered in the Meeting.

Sample abstract:

TRPC6 Channels Are Required for Proliferation, Migration and Invasion of Breast Cancer Cell Lines by Modulation of Orai1 and Orai3 Surface Exposure.

Isaac Jardin1, Raquel Diez-Bello1, Jose J.Lopez1, Pedro C. Redondo1, Gines M. Salido1, Tarik Smani2, Juan A. Rosado1.

1Cellular Physiology Research Group, Department of Physiology, Institute of Molecular Pathology Biomarkers, University of Extremadura, 10003 Caceres, Spain. 2Department of Medical Physiology and Biophysic, Institute of Biomedicine of Sevilla, 41013 Sevilla, Spain.

Transient receptor potential channels convey signaling information from a number of stimuli to a wide variety of cellular functions, mainly by inducing changes in cytosolic Ca2+ concentration (1). Different members of the TRPC, TRPM and TRPV subfamilies have been reported to play a role in tumorigenesis. Here we show that the estrogen receptor positive and triple negative breast cancer cell lines, MCF7 and MDA-MB-231, respectively, exhibit enhanced expression of the TRPC6 channel as compared to the non-tumoral MCF10A cell line. In vitro TRPC6 knockdown using shRNA impaired MCF7 and MDA-MB-231 cell proliferation, migration and invasion detected by BrdU incorporation, wound healing and Boyden chamber assays, respectively. Using RNAi-mediated TRPC6 silencing as well as overexpression of the pore-dead dominant-negative TRPC6 mutant we have found that TRPC6 plays a relevant role in the activation of store-operated Ca2+ entry in the breast cancer cell lines but not in non-tumoral breast cells. Finally, we have found that TRPC6 interacts with Orai1 and Orai3 in MCF7 and MDA-MB-231 cells and is required for the translocation of Orai1 and Orai3 to the plasma membrane in MDA-MB-231 and MCF7 cells, respectively, upon Ca2+ store depletion. These findings introduce a novel mechanism for the modulation of Ca2+ influx and the development of different cancer hallmarks in breast cancer cells.

  1. Liu X, Ong HL, Ambudkar I. (2018) TRP channel involvement in salivary glands-some good, some bad. Cells. 7(7): 74.

Keywords: MCF7; MDA-MB-231; Orai1; Orai3; TRPC6; store-operated calcium entry

Acknowledgements: supported by …